Gainesville is highly accessible by road, bisected by Interstate 75 to the west, and navigated internally by major state routes like US-441 (13th Street), State Road 24 (Waldo Road/Archer Road), and State Road 26 (University Avenue).
Public transit is provided by the Regional Transit System (RTS), which operates an extensive bus network heavily utilized by UF students (who ride fare-free with a student ID) and local residents. For air travel, the Gainesville Regional Airport (GNV) offers commercial flights connecting to major hubs like Atlanta, Charlotte, and Dallas/Fort Worth.
Gainesville is celebrated for its bicycle infrastructure, featuring numerous dedicated bike lanes and extensive paved trails. The Gainesville-Hawthorne State Trail is a notable 16-mile paved path stretching from the city through conservation lands. Rideshare services (Uber/Lyft) and scooters are ubiquitous, particularly around the university.